The biosynthesis of ribonucleic acid following infection with a ribonucleic acid virus.
نویسندگان
چکیده
The replication of deoxyribonucleic acid viruses is dependent on the synthesis of deoxyribonucleic acid, and increases in the activity of deoxyribonucleic acid nucleotidyltransferase (DNA polymerase) have been demonstrated in bacterial and mammalian cells infected with deoxyribonucleic acid viruses (1, 2). In contrast, several lines of evidence suggest that synthesis of DNA is not a prerequisite of the replication of RNA viruses. Thus DNA synthesis declines in “L” cells infected with the RNAcontaining Mengo virus (3) and inhibitors of DNA synthesis fail to interfere with the production of infective RNA viruses in HeLa cells (4, 5). These observations raise the question as to whether the synthesis of viral RNA uses existing host cell DNA and therefore is independent of DNA synthesis or is primed by the infecting RNA directly. A partial solution to this problem has been provided by the demonstration that there is a decline in the activity of DNA-primed RNA nucleotidyltransferase (RNA polymerase) in the nuclei of “L” cells infected with Mengo virus (3) and that poliovirus replication continues at an undiminished rate in “L” cells in the presence of concentrations of actinomycin D sufficient to abolish DNA-dependent synthesis of RNA (6). In this report, evidence is presented for the existence of independent RNA-primed and DNA-primed RNA nucleotidyltransferases in Krebs II ascites tumor cells. There was no difference in the activities of the DNA nucleotidyltransferase and DNAprimed RNA nucleotidyltransferase of uninfected cells and cells infected with the virus of mouse encephalomyocarditis (7). However, the levels of RNA-primed RNA nucleotidyltransferase activity increased in the infected cells and were significantly higher than in the controls. A preliminary report of this work has already appeared (8).
منابع مشابه
Foot-and-mouth disease virus-induced alterations of baby hamster kidney cell macromolecular biosynthesis: inhibition of ribonucleic acid methylation and stimulation of ribonucleic acid synthesis.
The kinetics of ribonucleic acid (RNA) and protein synthesis and RNA methylation were examined after foot-and-mouth disease virus (FMDV) infection of baby hamster kidney cells. The synthesis of RNA extracted from the whole cells was stimulated two- to threefold above the control level of synthesis. This increased rate was attributed to viral RNA synthesis. The inhibition of host RNA methylation...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 238 شماره
صفحات -
تاریخ انتشار 1963